Rabbit Monoclonal Antibody
The Ki-67 protein is a nuclear protein doublet, 345-395 kDa, playing a pivotal role in maintaining cell proliferation. In diagnostic histopathology and cell biology, the antibody has proven valuable for the demonstration of the Ki-67 antigen in normal and neoplastic cells, for example in soft-tissue sarcoma, prostatic adenocarcinoma, and breast carcinoma. The Ki-67 has been confirmed as a very powerful single prognostic factor for overall survival, with highly proliferative cases showing a much poorer outcome than tumors with low proliferation. In breast cancer, the proliferative index measured by Ki-67 immunoreactivity has both prognostic and predictive value.
Availability:
| Catalog No. | Contents | Volume |
| ILM0731-C01 | Ki-67 | 0,1 ml concentrate |
| ILM0731-C05 | Ki-67 | 0,5 ml concentrate |
| ILM0731-C1 | Ki-67 | 1,0 ml concentrate |
Intended use: For Research Use Only
Reactivity: Human, others not known
Clone: MXR002
Species of origin: Rabbit
Isotype: IgG
Control Tissue: Tonsil
Staining: Nuclear
Presentation: 1ml Ki-67 tissue culture supernatant contains 15mM Azide
Application and suggested dilutions:
Pre-treatment: Heat induced epitope retrieval in 10 mM citrate buffer pH6.0 for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections.
- Immunohistochemical staining of formalin-fixed, paraffin embedded tissue section (dilution up to 1:100-1:200)
The optimal dilution for a specific application should be determined by the investigator.
Note: Dilution of the antibody in 10% normal goat serum followed by a Goat anti-Rabbit secondary antibody-based detection is recommended.
Storage & Stability: Store at 2-8 °C. Do not use after expiration date printed on the vial.
Reference:
- Cheang, MU, Chia SK, Voduc D, et al. Ki-67 index, HER2 Status and Prognosis of Patients with luminal B Breast Cancer. J Natl Cancer inst, 2009, 101(10): 736-750
- Yerushalmi R, Woods R, Ravdin PM, et al. Ki-67 in Breast cancer: prognostic and predictive potential. Lancet Oncol, 2010, 11(2), 174-183