Mouse Monoclonal Antibody
It recognizes a 47kDa protein, which is identified as alpha sub-unit of Inhibin. It is a gonadal protein that preferentially suppresses the secretion of pituitary follicle-stimulating hormone (FSH). Inhibin comprises two subunits, Inhibin A and Inhibin B. Each subunit consists of the same subunit, covalently linked to 1 of 2 distinct subunits, – or -. Originally isolated from ovarian follicular fluid and characterized as a disulphide-linked dimeric glycoprotein, inhibin belongs to the transforming growth factor (TFG) superfamily. Antibodies against Inhibin are useful in making a differentiation between adrenal cortical tumors and renal cell carcinoma. Sex cord stromal tumors of the ovary as well as trophoblastic tumors also demonstrate cytoplasmic positivity. Inhibin antibody is also used to make the differential diagnosis of intra-uterine vs. ectopic pregnancy in endometrial curetting.
Availability:
| Catalog No. | Contents | Volume |
| ILM3623-C01 | Inhibin Alpha | 0,1 ml concentrate |
| ILM3623-C05 | Inhibin Alpha | 0,5 ml concentrate |
| ILM3623-C1 | Inhibin Alpha | 1,0 ml concentrate |
Intended use: For Research Use Only
Reactivity: Human
Clone: INHA/4265
Species of origin: Mouse
Isotype: IgG2a
Human Entrez Gene ID: 3623
Human SwissProt: P05111
Human chromosome location: 407506
Control Tissue: Prostate or testis
Staining: Nuclear and membranous
Immunogen: A synthetic peptide (around aa 73-96) of α-subunit of human Inhibin A protein (exact sequence is propietary)
Presentation: Bioreactor Concentrate with 0.05% Azide.
Application and suggested dilutions:
Pre-treatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0 is required for IHC staining on formalin-fixed, paraffin embedded tissue sections.
- Immunohistochemical staining of formalin-fixed, paraffin embedded tissue section (dilution 1:100-1:200).
The optimal dilution for a specific application should be determined by the investigator.
Note: Dilution of the antibody in 10% normal goat serum followed by a Goat anti-Mouse secondary antibody-based detection is recommended.
Storage & Stability: Store at 2-8 °C. Do not use after expiration date printed on the vial.
Reference:
- Balanathan, P., et al. (2009) Elevated level of inhibin- αsubunit is pro- tumourigenic and pro- metastatic and associatedwith extracapsular spread in advanced prostate cancer.British Journal of Cancer,100(11), 1784 1793.